CST6 Protein (AA 29-149) (His tag)
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- Target See all CST6 Proteins
- CST6 (Cystatin E/M (CST6))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 29-149
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CST6 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Top Product
- Discover our top product CST6 Protein
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Cystatin E/M (CST6) (AA 61-149) protein (GST tag)
CST6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) protein (GST tag)CST6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) (Active) protein (His tag)CST6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
Cystatin E/M (CST6) protein (Myc-DYKDDDDK Tag)CST6 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer( pH 8.0) containing 10 % glycerol, 1 mM DTT, 0.1M NaCl.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CST6 (Cystatin E/M (CST6))
- Alternative Name
- CST6 (CST6 Products)
- Synonyms
- CST6 Protein, 1110017E11Rik Protein, N28197 Protein, ichq Protein, cystatin E/M Protein, CST6 Protein, Cst6 Protein
- Background
- CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.9 kDa (142aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001314
- UniProt
- Q15828
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