NANS Protein (AA 1-359) (His tag)
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- Target See all NANS Proteins
- NANS (N-Acetylneuraminic Acid Synthase (NANS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-359
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NANS protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- NANS, 1-359aa, Human, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product NANS Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
- Storage
- 4 °C
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- Target
- NANS (N-Acetylneuraminic Acid Synthase (NANS))
- Alternative Name
- NANS (NANS Products)
- Synonyms
- MGC84329 Protein, SAS Protein, N-acetylneuraminate synthase L homeolog Protein, N-acetylneuraminate synthase Protein, nans.L Protein, NANS Protein, Nans Protein
- Background
- NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Sialic acid synthase, SAS, N acetylneuraminate synthase. NCBI no.: NP_061819
- Molecular Weight
- 42.4 kDa (379aa) confirmed by MALDI-TOF
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