CPOX Protein (AA 111-454) (His tag)

Catalog No. ABIN5852918

Product Details

Target: CPOX (Coproporphyrinogen Oxidase (CPOX))

Protein Type: Recombinant

Protein Characteristics: AA 111-454

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CPOX protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSTSLGRPE EEEDELAHRC SSFMAPPVTD LGELRRRPGD MKTKMELLIL ETQAQVCQAL AQVDGGANFS VDRWERKEGG GGISCVLQDG CVFEKAGVSI SVVHGNLSEE AAKQMRSRGK VLKTKDGKLP FCAMGVSSVI HPKNPHAPTI HFNYRYFEVE EADGNKQWWF GGGCDLTPTY LNQEDAVHFH RTLKEACDQH GPDLYPKFKK WCDDYFFIAH RGERRGIGGI FFDDLDSPSK EEVFRFVQSC ARAVVPSYIP LVKKHCDDSF TPQEKLWQQL RRGRYVEFNL LYDRGTKFGL FTPGSRIESI LMSLPLTARW EYMHSPSENS KEAEILEVLR HPRDWVR

Purity: > 95 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CPOX (Coproporphyrinogen Oxidase (CPOX))

Alternative Name: CPOX (CPOX Products)

Synonyms: CPO Protein, CPX Protein, Cpo Protein, cpo Protein, cb535 Protein, sb:cb535 Protein, wu:fc43a01 Protein, DDBDRAFT_0188158 Protein, DDBDRAFT_0231414 Protein, DDB_0188158 Protein, DDB_0231414 Protein, BcDNA:GM14838 Protein, CG3433 Protein, Dmel\\CG3433 Protein, l(2)k10617 Protein, l(2)k11018 Protein, coproporphyrinogen oxidase Protein, coproporphyrinogen III oxidase Protein, Coproporphyrinogen oxidase Protein, CPOX Protein, Cpox Protein, cpox Protein, hemF Protein, Coprox Protein, CNE00980 Protein, Shewana3_4102 Protein, HemF_3 Protein, Vapar_2685 Protein, Dd586_0781 Protein, BC1002_0909 Protein, Trad_0221 Protein, Bresu_2732 Protein, Galf_2657 Protein, BC1003_2365 Protein, Lbys_0733 Protein, Ftrac_1490 Protein, Astex_1139 Protein, BC1001_1038 Protein, Pedsa_0199 Protein, Fluta_2926 Protein, bgla_1g29750 Protein, Halhy_0288 Protein, Sphch_2673 Protein, Mesop_2259 Protein

Background: Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria. Recombinant human CPOX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 41.6 kDa (367aa) confirmed by MALDI-TOF

NCBI Accession: NP_000088

UniProt: P36551