SPA17 Protein (AA 1-151) (His tag)
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- Target See all SPA17 Proteins
- SPA17 (Sperm Autoantigenic Protein 17 (SPA17))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-151
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SPA17 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product SPA17 Protein
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Sperm Autoantigenic Protein 17 (SPA17) (AA 1-151) protein (GST tag)
SPA17 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Autoantigenic Protein 17 (SPA17) (AA 51-149) protein (GST tag)SPA17 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Autoantigenic Protein 17 (SPA17) (AA 1-146), (partial) protein (GST tag)SPA17 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SPA17 (Sperm Autoantigenic Protein 17 (SPA17))
- Alternative Name
- SPA17 (SPA17 Products)
- Synonyms
- spa17 Protein, MGC89454 Protein, SPA17 Protein, CT22 Protein, SP17 Protein, SP17-1 Protein, Sp17 Protein, sperm autoantigenic protein 17 Protein, sperm autoantigenic protein 17 L homeolog Protein, spa17 Protein, SPA17 Protein, spa17.L Protein, Spa17 Protein
- Background
- SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 19.8 kDa (174aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_059121
- UniProt
- Q15506
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