DNAJC24 Protein (AA 1-149) (His tag)
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- Target See all DNAJC24 Proteins
- DNAJC24 (DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-149
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DNAJC24 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN
- Purity
- > 90 % by SDS - PAGE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DNAJC24 (DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24))
- Alternative Name
- DNAJC24 (DNAJC24 Products)
- Synonyms
- DPH4 Protein, JJJ3 Protein, ZCSL3 Protein, 1700030A21Rik Protein, 2610027M02Rik Protein, AV066965 Protein, AW240712 Protein, Dph4 Protein, MmDjC7 Protein, Zcsl3 Protein, RGD1564710 Protein, DnaJ heat shock protein family (Hsp40) member C24 Protein, DNAJC24 Protein, Dnajc24 Protein
- Background
- DnaJ homolog subfamily C member 24, also known as DNAJC24, is a member of the ZCSL family of proteins and each contain one DPH-type zinc finger. This enzyme is involved in the synthesis of diphthamide, a protein found on translation elongation factor EF-2 that is the target of bacterial ADP-ribosylating toxins. DNAJC24 stimulates the ATPase activity of several Hsp70-type chaperones. It is detected in heart, brain, spleen, lung, liver, kidney and testis. Recombinant human DNAJC24 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 19.5kDa (172aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_859057
- UniProt
- Q6P3W2
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