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Maltose Binding Protein Protein (MBP) (AA 27-392) (His tag)

Recombinant Maltose Binding Protein protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN5853046

Quick Overview for Maltose Binding Protein Protein (MBP) (AA 27-392) (His tag) (ABIN5853046)

Target

See all Maltose Binding Protein (MBP) Proteins
Maltose Binding Protein (MBP)

Protein Type

Recombinant

Origin

  • 3
  • 1
  • 1
E. coli

Source

  • 5
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

> 95 % by SDS - PAGE
  • Protein Characteristics

    AA 27-392

    Purification tag / Conjugate

    This Maltose Binding Protein protein is labelled with His tag.

    Sequence

    MGSSHHHHHH SSGLVPRGSH MGSMKIEEGK LVIWINGDKG YNGLAEVGKK FEKDTGIKVT VEHPDKLEEK FPQVAATGDG PDIIFWAHDR FGGYAQSGLL AEITPDKAFQ DKLYPFTWDA VRYNGKLIAY PIAVEALSLI YNKDLLPNPP KTWEEIPALD KELKAKGKSA LMFNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NGPWAWSNID TSKVNYGVTV LPTFKGQPSK PFVGVLSAGI NAASPNKELA KEFLENYLLT DEGLEAVNKD KPLGAVALKS YEEELAKDPR IAATMENAQK GEIMPNIPQM SAFWYAVRTA VINAASGRQT VDEALKDAQT NSSSNNNNNN NNNNLGIEGR
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.1M NaCl, 10 % glycerol

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target

    Maltose Binding Protein (MBP)

    Alternative Name

    maltose Binding Protein

    Background

    Cytoplasmic maltose-binding protein, also known as MBP, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant E.coli MBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

    Molecular Weight

    44.9kDa (410aa), confirmed by MALDI-TOF

    NCBI Accession

    NP_418458

    UniProt

    P0AEX9
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