C1GALT1 Protein (AA 30-363) (His tag)

Catalog No. ABIN7280327

Product Details

Target: C1GALT1 (Core 1 Synthase, Glycoprotein-N-Acetylgalactosamine 3-beta-Galactosyltransferase, 1 (C1GALT1))

Protein Type: Recombinant

Protein Characteristics: AA 30-363

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This C1GALT1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSLLGEKVD TQPNVLHNDP HARHSDDNGQ NHLEGQMNFN ADSSQHKDEN TDIAENLYQK VRILCWVMTG PQNLEKKAKH VKATWAQRCN KVLFMSSEEN KDFPAVGLKT KEGRDQLYWK TIKAFQYVHE HYLEDADWFL KADDDTYVIL DNLRWLLSKY DPEEPIYFGR RFKPYVKQGY MSGGAGYVLS KEALKRFVDA FKTDKCTHSS SIEDLALGRC MEIMNVEAGD SRDTIGKETF HPFVPEHHLI KGYLPRTFWY WNYNYYPPVE GPGCCSDLAV SFHYVDSTTM YELEYLVYHL RPYGYLYRYQ PTLPERILKE ISQANKNEDT KVKLGNP

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: C1GALT1 (Core 1 Synthase, Glycoprotein-N-Acetylgalactosamine 3-beta-Galactosyltransferase, 1 (C1GALT1))

Alternative Name: C1GALT1 (C1GALT1 Products)

Synonyms: C1GALT Protein, T-synthase Protein, 2210410E06Rik Protein, AV284120 Protein, c1galt1 Protein, fa98f05 Protein, zgc:66485 Protein, wu:fa98f05 Protein, C1GALT1 Protein, C1GalT1-A Protein, Core 1 beta3-Gal-T1-A Protein, wu:fi11c09 Protein, zgc:153355 Protein, core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Protein, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 L homeolog Protein, core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 Protein, core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1b Protein, core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1a Protein, C1GALT1 Protein, LOC100496837.L Protein, C1galt1 Protein, c1galt1b Protein, c1galt1a Protein

Background: C1GALT1 generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from uDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis. Recombinant human C1GALT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 41.4 kDa (357aa), confirmed by MALDI-TOF

NCBI Accession: NP_064541

UniProt: Q9NS00