SPINK1 Protein (AA 24-79) (His tag)
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- Target See all SPINK1 Proteins
- SPINK1 (serine Peptidase Inhibitor, Kazal Type 1 (SPINK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-79
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SPINK1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSDSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product SPINK1 Protein
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serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (His tag)
SPINK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 1-79) protein (GST tag)SPINK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (GST tag)SPINK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-79) protein (His tag)Crystallography grade SPINK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) (AA 24-80) protein (His tag)Crystallography grade SPINK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
serine Peptidase Inhibitor, Kazal Type 1 (SPINK1) proteinSPINK1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SPINK1 (serine Peptidase Inhibitor, Kazal Type 1 (SPINK1))
- Alternative Name
- SPINK1 (SPINK1 Products)
- Synonyms
- PCTT Protein, PSTI Protein, Spink3 Protein, TATI Protein, TCP Protein, Psti Protein, SPINK1 Protein, p12 Protein, serine peptidase inhibitor, Kazal type 1 Protein, SPINK1 Protein, Spink1 Protein
- Background
- SPINK1 is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. Recombinant human SPINK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 8.6 kDa (79aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_003113
- UniProt
- P00995
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