RPAIN Protein (AA 1-106) (His tag)
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- Target See all RPAIN Proteins
- RPAIN (RPA Interacting Protein (RPAIN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-106
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RPAIN protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAESLRS PRRSLYKLVG SPPWKEAFRQ RCLERMRNSR DRLLNRYRQA GSSGPGNSQN SFLVQEVMEE EWNALQSVEN CPEDLAQLEE LIDMAVLEEI QQELINQGL
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product RPAIN Protein
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RPA Interacting Protein (RPAIN) (AA 1-106) protein (His tag)
RPAIN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
RPA Interacting Protein (RPAIN) (AA 1-219) protein (GST tag)RPAIN Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RPA Interacting Protein (RPAIN) (AA 1-219) protein (Strep Tag)Crystallography grade RPAIN Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
RPA Interacting Protein (RPAIN) (AA 1-219) protein (Strep Tag)Crystallography grade RPAIN Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RPAIN (RPA Interacting Protein (RPAIN))
- Alternative Name
- RPAIN (RPAIN Products)
- Synonyms
- HRIP Protein, RIP Protein, 2400006N03Rik Protein, RP23-83I13.5 Protein, Rip Protein, RGD1308492 Protein, RPAIN Protein, MGC153251 Protein, rpain Protein, xripalpha Protein, rip-B Protein, RPA interacting protein Protein, RPA interacting protein S homeolog Protein, RPA interacting protein L homeolog Protein, RPAIN Protein, Rpain Protein, rpain Protein, rpain.S Protein, rpain.L Protein
- Background
- RPA-interacting protein isoform d, also known as RPAIN, is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPAIN interacting protein has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Recombinant human RPAIN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.7 kDa (129aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_001153718
- UniProt
- Q86UA6
- Pathways
- Protein targeting to Nucleus
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