UPRT Protein (AA 1-309) (His tag)
-
- Target See all UPRT Proteins
- UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-309
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UPRT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMATELQC PDSMPCHNQQ VNSASTPSPE QLRPGDLILD HAGGNRASRA KVILLTGYAH SSLPAELDSG ACGGSSLNSE GNSGSGDSSS YDAPAGNSFL EDCELSRQIG AQLKLLPMND QIRELQTIIR DKTASRGDFM FSADRLIRLV VEEGLNQLPY KECMVTTPTG YKYEGVKFEK GNCGVSIMRS GEAMEQGLRD CCRSIRIGKI LIQSDEETQR AKVYYAKFPP DIYRRKVLLM YPILSTGNTV IEAVKVLIEH GVQPSVIILL SLFSTPHGAK SIIQEFPEIT ILTTEVHPVA PTHFGQKYFG TD
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product UPRT Protein
-
Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT) (AA 1-309) protein (His tag)
UPRT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT) (AA 1-309) protein (GST tag)UPRT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT) (AA 1-309) protein (Strep Tag)Crystallography grade UPRT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT) protein (Myc-DYKDDDDK Tag)UPRT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))
- Alternative Name
- uPRT (UPRT Products)
- Synonyms
- Gm774 Protein, FUR1 Protein, UPP Protein, uracil phosphoribosyltransferase Protein, uracil phosphoribosyltransferase homolog Protein, Uprt Protein, UPRT Protein
- Background
- UPRT is a uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (uMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. Recombinant human uPRT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.2 kDa (332aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_659489
- UniProt
- Q96BW1
- Pathways
- Ribonucleoside Biosynthetic Process
-
