TRIAP1 Protein (AA 1-76) (His tag)

Catalog No. ABIN5853375

Product Details

Target: TRIAP1 (TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-76

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TRIAP1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNSVGEA CTDMKREYDQ CFNRWFAEKF LKGDSSGDPC TDLFKRYQQC VQKAIKEKEI PIEGLEFMGH GKEKPENSS

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: TRIAP1 (TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1))

Alternative Name: TRIAP1 (TRIAP1 Products)

Synonyms: MDM35 Protein, P53CSV Protein, WF-1 Protein, 1810015M01Rik Protein, AU020874 Protein, AU043831 Protein, P53csv Protein, Wf-1 Protein, p53csv-A Protein, triap1 Protein, triap1a Protein, p53csv-B Protein, triap1-a Protein, triap1-b Protein, triap1b Protein, zgc:112025 Protein, TP53 regulated inhibitor of apoptosis 1 Protein, TP53 regulated inhibitor of apoptosis 1 S homeolog Protein, TP53 regulated inhibitor of apoptosis 1 L homeolog Protein, TRIAP1 Protein, Triap1 Protein, triap1.S Protein, triap1.L Protein, triap1 Protein

Background: TP53-regulated inhibitor of apoptosis 1, also known as TRIAP1, contains a p53-binding site within its second exon and the reduction of expression by small interfering RNA enhanced apoptosis, whereas overexpression protected cells from apoptosis caused by DNA damage. TRIAP1 is induced significantly when cells have a low level of genotoxic stresses, but not when DNA damage is severe. TRIAP1 can modulate apoptotic pathways through interaction with Hsp70 that probably inhibits activity of apoptosis protease activating factor-1. Recombinant human TRIAP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 11.2kDa (99aa), confirmed by MALDI-TOF

NCBI Accession: NP_057483

UniProt: O43715

Pathways: Negative Regulation of intrinsic apoptotic Signaling