TRIAP1 Protein (AA 1-76) (His tag)
Quick Overview for TRIAP1 Protein (AA 1-76) (His tag) (ABIN5853375)
Target
See all TRIAP1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-76
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Purification tag / Conjugate
- This TRIAP1 protein is labelled with His tag.
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Sequence
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MNSVGEA CTDMKREYDQ CFNRWFAEKF LKGDSSGDPC TDLFKRYQQC VQKAIKEKEI PIEGLEFMGH GKEKPENSS -
Purification
- > 90% by SDS-PAGE
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TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1) (AA 1-76) protein (Fc Tag)
custom-made TRIAP1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1) (AA 1-76) protein (Fc Tag)custom-made TRIAP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1) (AA 1-76) protein (His-IF2DI Tag)TRIAP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1) protein (Myc-DYKDDDDK Tag)TRIAP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- TRIAP1 (TP53 Regulated Inhibitor of Apoptosis 1 (TRIAP1))
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Alternative Name
- TRIAP1
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Background
- TP53-regulated inhibitor of apoptosis 1, also known as TRIAP1, contains a p53-binding site within its second exon and the reduction of expression by small interfering RNA enhanced apoptosis, whereas overexpression protected cells from apoptosis caused by DNA damage. TRIAP1 is induced significantly when cells have a low level of genotoxic stresses, but not when DNA damage is severe. TRIAP1 can modulate apoptotic pathways through interaction with Hsp70 that probably inhibits activity of apoptosis protease activating factor-1. Recombinant human TRIAP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 11.2 kDa (99aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_057483
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Pathways
- Negative Regulation of intrinsic apoptotic Signaling
Target
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