PABPN1 Protein (AA 119-306) (His tag)
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- Target See all PABPN1 Proteins
- PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 119-306
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PABPN1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSLEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSIEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGF AYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRAR YRARTTNYNS SRSRFYSGFN SRPRGRVYRG RARATSWYSP Y
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product PABPN1 Protein
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Poly A Binding Protein Nuclear 1 (PABPN1) (AA 2-306) protein (His tag)
Crystallography grade PABPN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
Poly A Binding Protein Nuclear 1 (PABPN1) (AA 2-302) protein (His tag)Crystallography grade PABPN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))
- Alternative Name
- PABPN1 (PABPN1 Products)
- Synonyms
- OPMD Protein, PAB2 Protein, PABII Protein, PABP-2 Protein, PABP2 Protein, Pabp3 Protein, mPABII Protein, ARABIDOPSIS POLY(A) BINDING 2 Protein, ATPAB2 Protein, F28A23.130 Protein, F28A23_130 Protein, POLY(A) BINDING PROTEIN 2 Protein, POLY(A)-BINDING PROTEIN 2 Protein, poly(A) binding protein 2 Protein, opmd Protein, pab2 Protein, pabp2 Protein, pabpii Protein, pabpn1 Protein, Pabpn1 Protein, Poly(A)-binding protein 2 Protein, fb19c03 Protein, wu:fb17e03 Protein, wu:fb18c06 Protein, wu:fb19c03 Protein, zgc:85979 Protein, zgc:92452 Protein, poly(A) binding protein nuclear 1 Protein, poly(A) binding protein, nuclear 1 Protein, poly(A) binding protein 2 Protein, poly(A) binding protein, nuclear 1 S homeolog Protein, poly(A) binding protein, nuclear 1 L homeolog Protein, polyadenylate-binding protein 2 Protein, poly(A) binding protein Pab2 Protein, PABPN1 Protein, Pabpn1 Protein, PAB2 Protein, pabpn1.S Protein, pabpn1.L Protein, LOC100728725 Protein, pab2 Protein, pabpn1 Protein
- Background
- PABPN1 is an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. Recombinant human PABPN1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 23.8 kDa (211aa)
- NCBI Accession
- NP_004634
- UniProt
- Q86U42
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