SNURF Protein (AA 1-71) (His tag)

Catalog No. ABIN5853504

Product Details

Target: SNURF (SNRPN Upstream Reading Frame (SNURF))

Protein Type: Recombinant

Protein Characteristics: AA 1-71

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SNURF protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMERARDR LHLRRTTEQH VPEVEVQVKR RRTASLSNQE CQLYPRRSQQ QQVPVVDFQA ELRQAFLAET PRGG

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 40 % glycerol, 1  mM DTT.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SNURF (SNRPN Upstream Reading Frame (SNURF))

Alternative Name: SNuRF (SNURF Products)

Synonyms: 2410045I01Rik Protein, Snrpn Protein, SNRPN upstream reading frame Protein, small nuclear ribonucleoprotein polypeptide N Protein, SNURF Protein, SNRPN Protein, Snurf Protein

Background: SNuRF is a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' uTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known. Recombinant human SNuRF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 10.8 kDa (94aa) confirmed by MALDI-TOF

NCBI Accession: NP_073715

UniProt: Q9Y675