Chromosome 7 Open Reading Frame 20 (C7orf20) (AA 1-327) protein (His tag)
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- Target See all Chromosome 7 Open Reading Frame 20 (C7orf20) Proteins
- Chromosome 7 Open Reading Frame 20 (C7orf20)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-327
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product C7orf20 Protein
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Chromosome 7 Open Reading Frame 20 (C7orf20) (AA 2-327) protein (His tag)
Crystallography grade C7orf20 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
Chromosome 7 Open Reading Frame 20 (C7orf20) (AA 2-327) protein (His tag)Crystallography grade C7orf20 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
Chromosome 7 Open Reading Frame 20 (C7orf20) protein (DYKDDDDK Tag)C7orf20 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Chromosome 7 Open Reading Frame 20 (C7orf20)
- Alternative Name
- GET4 (C7orf20 Products)
- Synonyms
- cee Protein, cgi-20 Protein, c7orf20 Protein, get4 Protein, C7orf20 Protein, CEE Protein, TRC35 Protein, 1110007L15Rik Protein, AW412535 Protein, Cee Protein, RGD1311660 Protein, C25H7ORF20 Protein, get4-a Protein, golgi to ER traffic protein 4 Protein, chromosome 7 open reading frame 20 Protein, Cee Protein, golgi to ER traffic protein 4 homolog (S. cerevisiae) Protein, golgi to ER traffic protein 4 S homeolog Protein, get4 Protein, Bm1_33940 Protein, LOC100136505 Protein, GET4 Protein, Get4 Protein, get4.S Protein
- Background
- GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Recombinant human GET4 protein, fused to His-tag at N-terminus, was expressed in E.coli .
- Molecular Weight
- 38.9 kDa (350aa)
- NCBI Accession
- NP_057033
- UniProt
- Q7L5D6
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