MED27 Protein (AA 1-311) (His tag)

Catalog No. ABIN5853665

Product Details

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Protein Type: Recombinant

Protein Characteristics: AA 1-311

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MED27 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADVINV SVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIAHFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDPV QDKTPLYSQL LQAYKWSNKL QYHAGLASGL LNQQSLKRSA NQMGVSAKRR PKAQPTTLVL PPQYVDDVIS RIDRMFPEMS IHLSRPNGTS AMLLVTLGKV LKVIVVMRSL FIDRTIVKGY NENVYTEDGK LDIWSKSNYQ VFQKVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27 (MED27 Products)

Synonyms: CRAP34 Protein, CRSP34 Protein, CRSP8 Protein, TRAP37 Protein, CG1245 Protein, Dmel\\CG1245 Protein, Med27 Protein, Trap37 Protein, dCRSP34 Protein, dCrsp34 Protein, dTRAP37 Protein, med27 Protein, crsp8 Protein, zgc:66302 Protein, MED27 Protein, DDBDRAFT_0216764 Protein, DDBDRAFT_0266847 Protein, DDB_0216764 Protein, DDB_0266847 Protein, 1500015J03Rik Protein, 2310042P07Rik Protein, AA682045 Protein, Crsp8 Protein, D2Ertd434e Protein, RGD1564993 Protein, DKFZp459I1912 Protein, CRSP complex subunit 8-B Protein, Xcrsp34 Protein, crap34 Protein, crsp34 Protein, crsp8-B Protein, med27-a Protein, med27-b Protein, trap37 Protein, mediator complex subunit 27 Protein, Mediator complex subunit 27 Protein, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa Protein, hypothetical protein Protein, mediator complex subunit 27 S homeolog Protein, MED27 Protein, med27 Protein, crsp8 Protein, Med27 Protein, med27.S Protein

Background: MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Recombinant human MED27 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 37.8 kDa (334aa)

NCBI Accession: NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha