HERC5 Protein (AA 681-1024) (His tag)
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- Target See all HERC5 Proteins
- HERC5 (Hect Domain and RLD 5 (HERC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 681-1024
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HERC5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product HERC5 Protein
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Hect Domain and RLD 5 (HERC5) (AA 681-1024) protein (His-IF2DI Tag)
HERC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Hect Domain and RLD 5 (HERC5) (AA 915-1024) protein (GST tag)HERC5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Hect Domain and RLD 5 (HERC5) (AA 1-1024) protein (Strep Tag)Crystallography grade HERC5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HERC5 (Hect Domain and RLD 5 (HERC5))
- Alternative Name
- HERC5 (HERC5 Products)
- Synonyms
- HERC5 Protein, CEB1 Protein, CEBP1 Protein, HECT and RLD domain containing E3 ubiquitin protein ligase 5 Protein, hect domain and RLD 5 Protein, HERC5 Protein
- Background
- HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 43 kDa (367aa)
- NCBI Accession
- NP_057407
- UniProt
- Q9UII4
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