HCV 1b NS5B Protein (AA 2634-2752) (His tag)
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- Target See all HCV 1b NS5B (HCV NS5b) products
- HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2634-2752
- Origin
- Hepatitis C Virus (HCV)
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HCV 1b NS5B protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPMGF SYDTRCFDST VTESDIRTEE AIYQCCDLDP QARVAIKSLT ERLYVGGPLT NSRGENCGYR RCRASGVLTT SCGNTLTCYI KARAACRAAG LQDCTMLVCG DDLVVICESA GVQED
- Purity
- > 90 % by SDS - PAGE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
- Alternative Name
- HCV-NS5b (HCV NS5b Products)
- Target Type
- Viral Protein
- Background
- Hepatitis C virus genotype 1 is a core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit and also regulates many host cellular functions such as signaling pathways and apoptosis. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Recombinant HCV(Hepatitis C Virus) NS5b protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 17.0 kDa (155aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_671491
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