BCCIP Protein (AA 1-314) (His tag)

Catalog No. ABIN5853793

Product Details

Target: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Protein Type: Recombinant

Protein Characteristics: AA 1-314

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BCCIP protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMASRS KRRAVESGVP QPPDPPVQRD EEEEKEVENE DEDDDDSDKE KDEEDEVIDE EVNIEFEAYS LSDNDYDGIK KLLQQLFLKA PVNTAELTDL LIQQNHIGSV IKQTDVSEDS NDDMDEDEVF GFISLLNLTE RKGTQCVEQI QELVLRFCEK NCEKSMVEQL DKFLNDTTKP VGLLLSERFI NVPPQIALPM YQQLQKELAG AHRTNKPCGK CYFYLLISKT FVEAGKNNSK KKPSNKKKAA LMFANAEEEF FYEKAILKFN YSVQEESDTC LGGKWSFDDV PMTPLRTVML IPGDKMNEIM DKLKEYLSV

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Alternative Name: BCCIP (BCCIP Products)

Synonyms: zgc:110272 Protein, mp:zf637-1-000774 Protein, BCCIP Protein, DKFZp459K2416 Protein, MGC82792 Protein, tok-1 Protein, MGC89311 Protein, TOK-1 Protein, TOK1 Protein, 1110013J05Rik Protein, BRCA2 and CDKN1A interacting protein Protein, BRCA2 and CDKN1A interacting protein L homeolog Protein, BCCIP Protein, bccip Protein, bccip.L Protein, Bccip Protein

Background: BCCIP was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Recombinant human BCCIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 38.6kDa(339aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_510868

UniProt: Q9P287