QTRTD1 Protein (AA 1-415) (His tag)

Catalog No. ABIN5853951

Product Details

Target: QTRTD1 (Queuine tRNA-Ribosyltransferase Domain Containing 1 (QTRTD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-415

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This QTRTD1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS

Purity: > 95 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate buffer saline (  pH 7.4) containing 30 % glycerol, 2  mM DTT, 1  mM EDTA, 0.1  mM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: QTRTD1 (Queuine tRNA-Ribosyltransferase Domain Containing 1 (QTRTD1))

Alternative Name: QTRTD1 (QTRTD1 Products)

Synonyms: 3110012M05Rik Protein, 4930470H18Rik Protein, AI648807 Protein, queuine tRNA-ribosyltransferase accessory subunit 2 Protein, queuine tRNA-ribosyltransferase accessory subunit 2 L homeolog Protein, QTRT2 Protein, qtrt2 Protein, qtrt2.L Protein, Qtrt2 Protein

Background: QTRTD1 interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GuN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine. Recombinant human QTRTD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 49.1kDa (438aa) confirmed by MALDI-TOF

NCBI Accession: NP_078914

UniProt: Q9H974

Pathways: Ribonucleoside Biosynthetic Process