NT5C3L Protein (AA 1-300) (His tag)
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- Target See all NT5C3L Proteins
- NT5C3L (5'-Nucleotidase, Cytosolic III-Like (NT5C3L))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-300
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NT5C3L protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product NT5C3L Protein
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5'-Nucleotidase, Cytosolic III-Like (NT5C3L) (AA 1-300) protein (His tag)
NT5C3L Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
5'-Nucleotidase, Cytosolic III-Like (NT5C3L) protein (Myc-DYKDDDDK Tag)NT5C3L Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. Phosphate Buffered Saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NT5C3L (5'-Nucleotidase, Cytosolic III-Like (NT5C3L))
- Alternative Name
- NT5C3B (NT5C3L Products)
- Synonyms
- NT5C3B Protein, NT5C3L Protein, 2610037D24Rik Protein, AI841843 Protein, C330027I04Rik Protein, Nt5c3l Protein, RP23-392I3.10 Protein, Nt5c3b-a Protein, Nt5c3b-b Protein, nt5c3l Protein, nt5c3l-A Protein, nt5c3l-b Protein, 5'-nucleotidase, cytosolic IIIB Protein, NT5C3B Protein, Nt5c3b Protein, nt5c3b.L Protein
- Background
- NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also it has weak activity for CMP. Recombinant human NT5C3B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 36.8 kDa(323aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_443167
- UniProt
- Q969T7
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