NFU1 Protein (AA 10-254) (His tag)
NFU1
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
1 image
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- Target See all NFU1 Proteins
- NFU1 (NFU1 Iron-Sulfur Cluster Scaffold (NFU1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 10-254
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NFU1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPV RYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENE ELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKG FEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product NFU1 Protein
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NFU1 Iron-Sulfur Cluster Scaffold (NFU1) (AA 10-254) protein (His tag)
NFU1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
NFU1 Iron-Sulfur Cluster Scaffold (NFU1) (AA 1-254) protein (GST tag)NFU1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 20 % glycerol 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NFU1 (NFU1 Iron-Sulfur Cluster Scaffold (NFU1))
- Alternative Name
- NFu1 (NFU1 Products)
- Synonyms
- HIRIP Protein, HIRIP5 Protein, MMDS1 Protein, NIFUC Protein, Nfu Protein, NifU Protein, 0610006G17Rik Protein, CGI-33 Protein, Hirip5 Protein, NFU1 iron-sulfur cluster scaffold Protein, NFU1 Protein, Nfu1 Protein
- Background
- NFu1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFu1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 29.9 kDa (268aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001002755
- UniProt
- Q9UMS0
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