PAX8 Protein (AA 1-287) (His tag)
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- Target See all PAX8 Proteins
- PAX8 (Paired Box 8 (PAX8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-287
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PAX8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMPHNSIR SGHGGLNQLG GAFVNGRPLP EVVRQRIVDL AHQGVRPCDI SRQLRVSHGC VSKILGRYYE TGSIRPGVIG GSKPKVATPK VVEKIGDYKR QNPTMFAWEI RDRLLAEGVC DNDTVPSVSS INRIIRTKVQ QPFNLPMDSC VATKSLSPGH TLIPSSAVTP PESPQSDSLG STYSINGLLG IAQPGSDKRK MDDSDQDSCR LSIDSQSSSS GPRKHLRTDA FSQHHLEPLE CPFERQHYPE AYASPSHTKG EQEVNTLAMP MATPPTPPTA RPGASPTPAC
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PAX8 Protein
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Paired Box 8 (PAX8) (AA 141-250) protein (GST tag)
PAX8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Paired Box 8 (PAX8) (AA 243-450) protein (His tag)PAX8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Paired Box 8 (PAX8) (AA 1-450) protein (GST tag)PAX8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Paired Box 8 (PAX8) (AA 300-377) protein (GST tag)PAX8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Paired Box 8 (PAX8) (AA 1-450) protein (Strep Tag)Crystallography grade PAX8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Paired Box 8 (PAX8) (AA 1-457) protein (Strep Tag)Crystallography grade PAX8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Paired Box 8 (PAX8) protein (Myc-DYKDDDDK Tag)PAX8 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4) containing 20 % glycerol , 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PAX8 (Paired Box 8 (PAX8))
- Alternative Name
- PAX8 (PAX8 Products)
- Synonyms
- PAX8 Protein, XPax-8 Protein, XPax8 Protein, pax-8 Protein, PAX-8 Protein, Pax-8 Protein, paired box 8 Protein, paired box 8 L homeolog Protein, PAX8 Protein, pax8 Protein, Pax8 Protein, pax8.L Protein
- Background
- Paired box protein Pax-8 isoform PAX8E, also known as PAX8, is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. PAX8complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2). Recombinant human PAX8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 33.4 kDa (310aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_054698
- UniProt
- Q06710
- Pathways
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation
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