MTHFD2 Protein (AA 30-350) (His tag)

Catalog No. ABIN5778737

Product Details

Target: MTHFD2 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))

Protein Type: Recombinant

Protein Characteristics: AA 30-350

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MTHFD2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSLAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN

Purity: > 95 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: MTHFD2 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))

Alternative Name: MTHFD2 (MTHFD2 Products)

Synonyms: nmdmc Protein, AW558851 Protein, NMDMC Protein, wu:fb38b11 Protein, wu:fe12e11 Protein, zgc:91891 Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase L homeolog Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like S homeolog Protein, methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase Protein, mthfd2.L Protein, MTHFD2 Protein, mthfd2 Protein, mthfd2l.S Protein, Mthfd2 Protein

Background: MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 37.2 kDa (344aa) confirmed by MALDI-TOF

NCBI Accession: NP_006627

UniProt: P13995