RPRD1B Protein (AA 1-326) (His tag)

Catalog No. ABIN5854183

Product Details

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Protein Type: Recombinant

Protein Characteristics: AA 1-326

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPRD1B protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSFSES ALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEF TREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLK RTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKI TDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVT QVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Alternative Name: RPRD1B (RPRD1B Products)

Synonyms: fb23h08 Protein, zgc:55687 Protein, wu:fb23h08 Protein, rprd1ba Protein, MGC130705 Protein, C13H20ORF77 Protein, C20orf77 Protein, CREPT Protein, NET60 Protein, dJ1057B20.2 Protein, 2610304G08Rik Protein, 2810446G03Rik Protein, Crept Protein, RGD1304782 Protein, regulation of nuclear pre-mRNA domain containing 1B Protein, regulation of nuclear pre-mRNA domain containing 1B L homeolog Protein, rprd1b Protein, rprd1b.L Protein, RPRD1B Protein, Rprd1b Protein

Background: RPRD1B also known as regulation of nuclear pre-mRNA domain-containing protein 1B. This protein interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. It promotes cell proliferation. Recombinant human RPRD1B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 39.3kDa (349aa) confirmed by MALDI-TOF

NCBI Accession: NP_067038

UniProt: Q9NQG5