CA13 Protein (AA 1-262) (His tag)

Catalog No. ABIN5854197

Product Details

Target: CA13 (Carbonic Anhydrase XIII (CA13))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-262

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CA13 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSP MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH

Purity: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CA13 (Carbonic Anhydrase XIII (CA13))

Alternative Name: CA13 (CA13 Products)

Synonyms: 2310075C21Rik Protein, Ca13 Protein, CAXIII Protein, car13 Protein, RGD1560453 Protein, car3 Protein, CA13 Protein, carbonic anhydrase 13 Protein, carbonic anhydrase XIII L homeolog Protein, carbonic anhydrase XIII Protein, Car13 Protein, CA13 Protein, ca13.L Protein, ca13 Protein, LOC100152714 Protein, Ca13 Protein, LOC101113860 Protein

Background: CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 31.8kDa (285aa) confirmed by MALDI-TOF

NCBI Accession: NP_940986

UniProt: Q8N1Q1