UBE2E3 Protein (AA 1-207) (His tag)

Catalog No. ABIN5854295

Product Details

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Protein Type: Recombinant

Protein Characteristics: AA 1-207

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2E3 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSDRQR SDDESPSTSS GSSDADQRDP AAPEPEEQEE RKPSATQQKK NTKLSSKTTA KLSTSAKRIQ KELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS SDYPFKPPKV TFRTRIYHCN INSQGVICLD ILKDNWSPAL TISKVLLSIC SLLTDCNPAD PLVGSIATQY LTNRAEHDRI ARQWTKRYAT

Purity: > 80 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: UBE2E3 (UBE2E3 Products)

Synonyms: UBCH9 Protein, UbcM2 Protein, UbcD2 Protein, wu:fb26h04 Protein, zgc:55923 Protein, zgc:76906 Protein, ubc15 Protein, ubch9 Protein, ubcm2 Protein, xubc15 Protein, Ubce4 Protein, ubcM2 Protein, ube2e3 Protein, ubiquitin conjugating enzyme E2 E3 Protein, ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2E 3 Protein, Ubiquitin-conjugating enzyme E2 E3 Protein, ubiquitin-conjugating enzyme E2 E3 Protein, ubiquitin conjugating enzyme E2 E3 L homeolog Protein, ubiquitin-conjugating enzyme UBE2E3 Protein, UBE2E3 Protein, ube2e3 Protein, Ube2e3 Protein, ub2e3 Protein, LOC100280942 Protein, LOC100282225 Protein, PTRG_01263 Protein, ube2e3.L Protein, LOC780737 Protein

Background: UBE2E3 also known as Ubiquitin-conjugating enzyme E2 E3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell. Recombinant human UBE2E3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 25.3kDa (230aa), confirmed by MALDI-TOF

NCBI Accession: NP_006348

UniProt: Q969T4