IDS Protein (AA 26-550) (His tag)
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- Target See all IDS Proteins
- IDS (Iduronate 2-Sulfatase (IDS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-550
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This IDS protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- SETQANSTTD ALNVLLIIVD DLRPSLGCYG DKLVRSPNID QLASHSLLFQ NAFAQQAVCA PSRVSFLTGR RPDTTRLYDF NSYWRVHAGN FSTIPQYFKE NGYVTMSVGK VFHPGISSNH TDDSPYSWSF PPYHPSSEKY ENTKTCRGPD GELHANLLCP VDVLDVPEGT LPDKQSTEQA IQLLEKMKTS ASPFFLAVGY HKPHIPFRYP KEFQKLYPLE NITLAPDPEV PDGLPPVAYN PWMDIRQRED VQALNISVPY GPIPVDFQRK IRQSYFASVS YLDTQVGRLL SALDDLQLAN STIIAFTSDH GWALGEHGEW AKYSNFDVAT HVPLIFYVPG RTASLPEAGE KLFPYLDPFD SASQLMEPGR QSMDLVELVS LFPTLAGLAG LQVPPRCPVP SFHVELCREG KNLLKHFRFR DLEEDPYLPG NPRELIAYSQ YPRPSDIPQW NSDKPSLKDI KIMGYSIRTI DYRYTVWVGF NPDEFLANFS DIHAGELYFV DSDPLQDHNM YNDSQGGDLF QLLMPLEHHH HHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product IDS Protein
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Iduronate 2-Sulfatase (IDS) (AA 38-137) protein (GST tag)
IDS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Iduronate 2-Sulfatase (IDS) (AA 1-312) protein (GST tag)IDS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Iduronate 2-Sulfatase (IDS) (AA 34-455) protein (His tag)Crystallography grade IDS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Iduronate 2-Sulfatase (IDS) (AA 456-550) protein (His tag)Crystallography grade IDS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Iduronate 2-Sulfatase (IDS) (AA 36-552) protein (His tag)Crystallography grade IDS Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Iduronate 2-Sulfatase (IDS) proteinIDS Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- IDS (Iduronate 2-Sulfatase (IDS))
- Alternative Name
- IDS (IDS Products)
- Synonyms
- mps2 Protein, sids Protein, zgc:158245 Protein, MPS2 Protein, SIDS Protein, AW214631 Protein, iduronate 2-sulfatase Protein, IDS Protein, CpipJ_CPIJ004938 Protein, ids Protein, Ids Protein
- Background
- IDS, as known as iduronate 2-sulfatase isoform, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O-sulfate and N-salfate esters from a varity of substrates. This protein is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate. It hydrolyzes the 2-sulfate group of the IDS units of the GAG. Recombinant human IDS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 60.3kDa (533aa) 50-70kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_000193
- UniProt
- P22304
- Pathways
- Glycosaminoglycan Metabolic Process
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