IDS Protein (His tag)

Catalog No. ABIN7317906

Product Details

Target: IDS (Iduronate 2-Sulfatase (IDS))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IDS protein is labelled with His tag.

Purpose: Recombinant Human Iduronate 2-Sulfatase/IDS Protein (His Tag)(Active)

Sequence: Met 1-Pro 550

Characteristics: A DNA sequence encoding human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag.

Purity: > 87 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).The specific activity is > 1.0 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: Iduronate 2-Sulfatase/IDS (IDS Products)

Synonyms: mps2 Protein, sids Protein, zgc:158245 Protein, MPS2 Protein, SIDS Protein, AW214631 Protein, iduronate 2-sulfatase Protein, IDS Protein, CpipJ_CPIJ004938 Protein, ids Protein, Ids Protein

Background:

Background: Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.

Synonym: IDS,MPS2,SIDS

Molecular Weight: 61 kDa

NCBI Accession: NP_000193

Pathways: Glycosaminoglycan Metabolic Process