RNASE3 Protein (AA 28-160) (His tag)
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- Target See all RNASE3 (ECP) Proteins
- RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-160
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This RNASE3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH
- Purity
- > 95 % by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product ECP Protein
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Eosinophil Cationic Protein (ECP) (AA 28-160) protein (His tag)
Crystallography grade ECP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Eosinophil Cationic Protein (ECP) proteinECP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Eosinophil Cationic Protein (ECP) proteinECP Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))
- Alternative Name
- RNASE3 (ECP Products)
- Synonyms
- ECP Protein, RNS3 Protein, ribonuclease A family member 3 Protein, RNASE3 Protein
- Background
- RNASE3, also known as Ribonuclease RNase A family 3, located in the eosinophil primary matrix. It released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. It is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Recombinant human RNASE3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 16.6kDa (142aa) 18-28KDa (SDS-PAGE under reducing conditions.)
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