SRSF1 Protein (AA 1-248) (His tag)
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- Target See all SRSF1 Proteins
- SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-248
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This SRSF1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product SRSF1 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 100 mM KCl, 1 mM DTT, 0.2 mM EDTA, 40 % Glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))
- Alternative Name
- SRSF1 (SRSF1 Products)
- Synonyms
- ASF Protein, SF2 Protein, SF2p33 Protein, SFRS1 Protein, SRp30a Protein, 1110054N12Rik Protein, 5730507C05Rik Protein, 6330415C05Rik Protein, AI482334 Protein, AW491331 Protein, Asf Protein, Sf2 Protein, Sfrs1 Protein, DKFZp469K2419 Protein, sfrs1 Protein, sfrs1b Protein, wu:fb80g05 Protein, zgc:111894 Protein, zgc:65898 Protein, zgc:76897 Protein, asf Protein, sf2 Protein, sf2p33 Protein, srp30a Protein, sfrs1a Protein, sfrs1l Protein, wu:fb52g10 Protein, wu:fb97g12 Protein, zgc:66146 Protein, serine and arginine rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1b Protein, serine/arginine-rich splicing factor 1 L homeolog Protein, serine/arginine-rich splicing factor 1a Protein, SRSF1 Protein, Srsf1 Protein, srsf1b Protein, srsf1.L Protein, srsf1a Protein
- Background
- SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation. Recombinant human SRSF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 28.5kDa (254aa) 28-40KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_008855
- UniProt
- Q07955
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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