CSTF1 Protein (AA 1-431) (His tag)

Catalog No. ABIN5854876

Product Details

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-431

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CSTF1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 1  mM DTT, 20 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Alternative Name: CSTF1 (CSTF1 Products)

Synonyms: fc22d10 Protein, zgc:66207 Protein, zgc:77560 Protein, wu:fc22d10 Protein, wu:fd16h09 Protein, MGC53063 Protein, MGC69421 Protein, MGC83790 Protein, 1700057K18Rik Protein, AI788832 Protein, CstF-50 Protein, CstFp50 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1 Protein, cleavage stimulation factor subunit 1 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa Protein, cstf1 Protein, cstf1.S Protein, CSTF1 Protein, cstf1.L Protein, Cstf1 Protein

Background: CSTF1, also known as cleavage stimulation factor subunit 1, is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. It is functioning with cleavage-polyadenylation specificity factor, two cleavage factors, and poly(A)+ polymerase. It is involved in the 3' end processing of messenger RNA precursors. Recombinant human CSTF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 49.1kDa (437aa) 40-57kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_001028693

UniProt: Q05048