CRYGD Protein (AA 1-174) (His tag)
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- Target See all CRYGD Proteins
- CRYGD (Crystallin, gamma D (CRYGD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-174
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CRYGD protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGKITFY EDRGFQGRHY ECSTDHSNLQ PYFSRCNSVR VDSGCWMLYE QPNFTGCQYF LRRGDYPDYQ QWMGFSDSVR SCRLIPHAGS HRIRLYEREE YRGQMIEFTE DCPSLQDRFH FNEIYSLNVL EGCWVLYDMT NYRGRQYLLR PGEYRRYHDW GAMNARVGSL RRVMDFY
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product CRYGD Protein
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Crystallin, gamma D (CRYGD) (AA 1-174) protein (His tag)
CRYGD Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Crystallin, gamma D (CRYGD) (AA 1-174) protein (GST tag)CRYGD Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Crystallin, gamma D (CRYGD) (AA 75-174) protein (GST tag)CRYGD Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Crystallin, gamma D (CRYGD) (AA 2-174) protein (His tag)Crystallography grade CRYGD Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Crystallin, gamma D (CRYGD) (AA 2-174) protein (His tag)Crystallography grade CRYGD Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Crystallin, gamma D (CRYGD) protein (Myc-DYKDDDDK Tag)CRYGD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl( pH 8.0) containing 0.1M NaCl, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CRYGD (Crystallin, gamma D (CRYGD))
- Alternative Name
- Crygd (CRYGD Products)
- Synonyms
- ccp Protein, caca Protein, cca3 Protein, cryg4 Protein, cry-g-d Protein, MGC85594 Protein, CRYGD Protein, CACA Protein, CCA3 Protein, CCP Protein, CRYG4 Protein, CTRCT4 Protein, PCC Protein, cry-g-D Protein, Aey4 Protein, Cryg-1 Protein, DGcry-1 Protein, Lop12 Protein, Cryg4 Protein, Len Protein, crystallin gamma D L homeolog Protein, crystallin gamma D Protein, crystallin, gamma D Protein, crygd.L Protein, CRYGD Protein, Crygd Protein
- Background
- Crygd, also known as gamma-crystalin D, belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in Crygd are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant mouse Crygd protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.5 kDa (197aa) Confirmed by MALDI-TOF
- NCBI Accession
- NP_031802
- UniProt
- P04342
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