CRYGD Protein (AA 1-174) (His tag)

Catalog No. ABIN5854999

This Recombinant CRYGD protein is expressed in Escherichia coli (E. coli).

Quick Overview for CRYGD Protein (AA 1-174) (His tag) (ABIN5854999)

Target: CRYGD (Crystallin, gamma D (CRYGD))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-174

Purification tag / Conjugate: This CRYGD protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGKITFY EDRGFQGRHY ECSTDHSNLQ PYFSRCNSVR VDSGCWMLYE QPNFTGCQYF LRRGDYPDYQ QWMGFSDSVR SCRLIPHAGS HRIRLYEREE YRGQMIEFTE DCPSLQDRFH FNEIYSLNVL EGCWVLYDMT NYRGRQYLLR PGEYRRYHDW GAMNARVGSL RRVMDFY

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CRYGD (Crystallin, gamma D (CRYGD))

Alternative Name: Crystallin gamma D/CRYGD

Background: Crygd, also known as gamma-crystalin D, belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in Crygd are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant mouse Crygd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 23.5 kDa (197aa) Confirmed by MALDI-TOF

NCBI Accession: NP_031802