TPSAB1 Protein (AA 31-275) (His tag)
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- Target See all TPSAB1 Proteins
- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-275
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This TPSAB1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- IVGGQEAPRS KWPWQVSLRV HGPYWMHFCG GSLIHPQWVL TAAHCVGPDV KDLAALRVQL REQHLYYQDQ LLPVSRIIVH PQFYTAQIGA DIALLELEEP VNVSSHVHTV TLPPASETFP PGMPCWVTGW GDVDNDERLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD DVRIVRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWGEGCAQ PNRPGIYTRV TYYLDWIHHY VPKKPHHHHH H
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product TPSAB1 Protein
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Tryptase alpha/beta 1 (TPSAB1) (AA 38-282) protein (His tag)
TPSAB1 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Tryptase alpha/beta 1 (TPSAB1) (AA 1-275) protein (GST tag)TPSAB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Tryptase alpha/beta 1 (TPSAB1) (AA 19-275) protein (His tag)TPSAB1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 20 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
- Alternative Name
- TPSAB1 (TPSAB1 Products)
- Synonyms
- TPS1 Protein, TPS2 Protein, TPSB1 Protein, PMCT7 Protein, tpsab1 Protein, tryptaseB Protein, tryptaseC Protein, MMCP-7 Protein, Mcp-7 Protein, Mcp7 Protein, Mcpt7 Protein, rMCP-7 Protein, AV011504 Protein, MMCP-6 Protein, Mcp-6 Protein, Mcp6 Protein, Mcpt6 Protein, tryptase alpha/beta 1 Protein, tryptase Protein, tryptase beta 2 (gene/pseudogene) Protein, tryptase beta 2 Protein, TPSAB1 Protein, MCT7 Protein, tpsab1 Protein, TPSB2 Protein, Tpsab1 Protein, Tpsb2 Protein
- Background
- TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 28.2kDa (251aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_003285
- UniProt
- P20231
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