DDT Protein (AA 21-248) (His tag)
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- Target See all DDT Proteins
- DDT (D-Dopachrome Tautomerase (DDT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-248
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DDT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product DDT Protein
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D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)
DDT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT Origin: Dog Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His tag)DDT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His tag)DDT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (GST tag)DDT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT Origin: Mouse Host: Yeast Recombinant > 90 % SDS
D-Dopachrome Tautomerase (DDT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DDT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
D-Dopachrome Tautomerase (DDT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DDT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DDT (D-Dopachrome Tautomerase (DDT))
- Alternative Name
- D-Dopachrome Decarboxylase (DDT Products)
- Synonyms
- ddt-b Protein, zgc:86714 Protein, wu:fb49f11 Protein, C78655 Protein, DDT Protein, ddt Protein, ddt-a Protein, DDCT Protein, D-dopachrome tautomerase L homeolog Protein, D-dopachrome tautomerase Protein, D-dopachrome tautomerase S homeolog Protein, ddt.L Protein, DDT Protein, ddt Protein, Ddt Protein, ddt.S Protein
- Background
- Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihyroxyindole(DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.5 kDa (141aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_034157
- UniProt
- O35215
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