TUBG1 Protein (AA 1-451) (His tag)

Catalog No. ABIN5855121

Product Details

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Protein Type: Recombinant

Protein Characteristics: AA 1-451

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This TUBG1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE QHHHHHH

Purity: > 85 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl (  pH 8.0) containing 40 % glycerol, 0.1M NaCl, 2  mM DTT, 50  mM imidazole.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Alternative Name: TUBG1 (TUBG1 Products)

Synonyms: GCP-1 Protein, TUBG Protein, TUBGCP1 Protein, TUBC Protein, TUBG3 Protein, gamma-tubulin Protein, Tubg2 Protein, xgam Protein, 1500010O08Rik Protein, AI451582 Protein, AI503389 Protein, Tubg Protein, tubgl Protein, zgc:55894 Protein, tubulin gamma 1 Protein, gamma-tubulin 1 Protein, gamma-tubulin Protein, tubulin gamma 1 L homeolog Protein, Tubulin gamma chain Protein, tubulin, gamma 1 Protein, tubulin gamma-1 chain Protein, TUBG1 Protein, tubg1 Protein, tubg1.L Protein, tbg-1 Protein, Tubg1 Protein, LOC101686824 Protein, LOC101103906 Protein

Background: TUBG1, also known as tubulin gamma-1 chain, is a member of the tubulin superfamily. This protein is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. It is the pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. It is required for microtubule formation and progression of the cell cycle. Also, this protein is interacts with GCP2, GCP3, and B9D2. The interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Recombinant human TUBG1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 51.9kDa (457aa) 50-70kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001061

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase