KPNB1 Protein (AA 1-876) (His tag)

Catalog No. ABIN7314434

Product Details

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-876

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This KPNB1 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human KPNB1 Protein is produced by E.coli expression system and the target gene encoding Met1-Ala876 is expressed with a 6His tag at the N-terminus.

Sequence: MNHKVHHHHH HMELITILEK TVSPDRLELE AAQKFLERAA VENLPTFLVE LSRVLANPGN SQVARVAAGL QIKNSLTSKD PDIKAQYQQR WLAIDANARR EVKNYVLQTL GTETYRPSSA SQCVAGIACA EIPVNQWPEL IPQLVANVTN PNSTEHMKES TLEAIGYICQ DIDPEQLQDK SNEILTAIIQ GMRKEEPSNN VKLAATNALL NSLEFTKANF DKESERHFIM QVVCEATQCP DTRVRVAALQ NLVKIMSLYY QYMETYMGPA LFAITIEAMK SDIDEVALQG IEFWSNVCDE EMDLAIEASE AAEQGRPPEH TSKFYAKGAL QYLVPILTQT LTKQDENDDD DDWNPCKAAG VCLMLLATCC EDDIVPHVLP FIKEHIKNPD WRYRDAAVMA FGCILEGPEP SQLKPLVIQA MPTLIELMKD PSVVVRDTAA WTVGRICELL PEAAINDVYL APLLQCLIEG LSAEPRVASN VCWAFSSLAE AAYEAADVAD DQEEPATYCL SSSFELIVQK LLETTDRPDG HQNNLRSSAY ESLMEIVKNS AKDCYPAVQK TTLVIMERLQ QVLQMESHIQ STSDRIQFND LQSLLCATLQ NVLRKVQHQD ALQISDVVMA SLLRMFQSTA GSGGVQEDAL MAVSTLVEVL GGEFLKYMEA FKPFLGIGLK NYAEYQVCLA AVGLVGDLCR ALQSNIIPFC DEVMQLLLEN LGNENVHRSV KPQILSVFGD IALAIGGEFK KYLEVVLNTL QQASQAQVDK SDYDMVDYLN ELRESCLEAY TGIVQGLKGD QENVHPDVML VQPRVEFILS FIDHIAGDED HTDGVVACAA GLIGDLCTAF GKDVLKLVEA RPMIHELLTE GRRSKTNKAK TLATWATKEL RKLKNQA

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in a 0.2 μm filtered solution of 20  mM Tris, 1  mM DTT, 30 % glycerol, 0.1M Nacl,  pH 8.0.

Handling Advice: Please minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Alternative Name: KPNB1 (KPNB1 Products)

Synonyms: Afu1g15720 Protein, AO090005001133 Protein, imb1 Protein, impnb Protein, ipo1 Protein, ipob Protein, ntf97 Protein, IMB1 Protein, IPO1 Protein, IPOB Protein, Impnb Protein, NTF97 Protein, AA409963 Protein, importin beta-1 subunit Protein, Importin beta-1 subunit Protein, karyopherin (importin) beta 1 L homeolog Protein, importin beta 1 Protein, karyopherin subunit beta 1 Protein, karyopherin (importin) beta 1 Protein, AFUA_1G15720 Protein, AOR_1_1974174 Protein, Bm1_57540 Protein, PMAA_065830 Protein, AFLA_083750 Protein, TSTA_085870 Protein, BDBG_07509 Protein, MCYG_00039 Protein, kpnb1.L Protein, LOC100533387 Protein, KPNB1 Protein, Kpnb1 Protein

Gene ID: 3837

UniProt: Q14974

Pathways: Protein targeting to Nucleus