SEMA3A Protein (AA 26-546) (DYKDDDDK Tag,His tag)
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- Target See all SEMA3A Proteins
- SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-546
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This SEMA3A protein is labelled with DYKDDDDK Tag,His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human Semaphorin 3A Protein is produced by mammalian expression system and the target gene encoding Lys26-Phe546 is expressed with a 6His-Flag tag at the C-terminus.
- Sequence
- GSGSHHHHHH DYKDDDDKKN NVPRLKLSYK EMLESNNVIT FNGLANSSSY HTFLLDEERS RLYVGAKDHI FSFDLVNIKD FQKIVWPVSY TRRDECKWAG KDILKECANF IKVLKAYNQT HLYACGTGAF HPICTYIEIG HHPEDNIFKL ENSHFENGRG KSPYDPKLLT ASLLIDGELY SGTAADFMGR DFAIFRTLGH HHPIRTEQHD SRWLNDPKFI SAHLISESDN PEDDKVYFFF RENAIDGEHS GKATHARIGQ ICKNDFGGHR SLVNKWTTFL KARLICSVPG PNGIDTHFDE LQDVFLMNFK DPKNPVVYGV FTTSSNIFKG SAVCMYSMSD VRRVFLGPYA HRDGPNYQWV PYQGRVPYPR PGTCPSKTFG GFDSTKDLPD DVITFARSHP AMYNPVFPMN NRPIVIKTDV NYQFTQIVVD RVDAEDGQYD VMFIGTDVGT VLKVVSIPKE TWYDLEEVLL EEMTVFREPT AISAMELSTK QQQLYIGSTA GVAQLPLHRC DIYGKACAEC CLARDPYCAW DGSACSRYF
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Top Product
- Discover our top product SEMA3A Protein
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Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-772) protein (His tag)
SEMA3A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 672-770) protein (GST tag)SEMA3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) protein (Fc Tag)SEMA3A Origin: Mouse Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-771) protein (His tag)Crystallography grade SEMA3A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A) (AA 21-772) protein (His tag)Crystallography grade SEMA3A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
- Alternative Name
- SEMA3A (SEMA3A Products)
- Synonyms
- COLL1 Protein, HH16 Protein, Hsema-I Protein, Hsema-III Protein, SEMA1 Protein, SEMAD Protein, SEMAIII Protein, SEMAL Protein, SemD Protein, coll-1 Protein, Semad Protein, COLL-1 Protein, SEMA3A Protein, Dsema3a Protein, Sema3A Protein, sema1a Protein, sema3a1 Protein, semaZ1a Protein, sema1b Protein, sema3a2 Protein, semaZ1b Protein, semaphorin 3A Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A Protein, Sema-3a Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab Protein, SEMA3A Protein, Sema3a Protein, Sema-3a Protein, sema3aa Protein, sema3ab Protein
- Gene ID
- 10371
- UniProt
- Q14563
- Pathways
- Regulation of Cell Size
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