EPO Protein (His tag)
Quick Overview for EPO Protein (His tag) (ABIN6122629)
Target
See all EPO ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This EPO protein is labelled with His tag.
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Purpose
- Monkey Erythropoietin (EPO) Protein
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Sequence
- APP RLICDSRVLE RYLLEAKEAE NVTMGCSESC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQAVLAN SSQPFEPLQL HMDKAISGLR SITTLLRALG AQQEAISLPD AASAAPLRTI TADTFCKLFR VYSNFLRGKL KLYTGEACRR GDR
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Endotoxin Level
- < 1.0 EU per 1 μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 27-192) (Active) protein (His tag)EPO Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Erythropoietin (EPO) (AA 27-192) (Active) protein (His tag)EPO Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- EPO (Erythropoietin (EPO))
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Alternative Name
- Erythropoietin
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Target Type
- Hormone
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Molecular Weight
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Calculated MW: 22.1 kDa
Observed MW (SDS-PAGE): 27 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.Gene ID
- 719294
UniProt
- Q28513
Pathways
- JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
Target
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