Periostin Protein (POSTN) (His tag)

Catalog No. ABIN623731

Product Details

Target: Periostin (POSTN)

Protein Type: Recombinant

Origin: Human

Host: Please inquire

Purification tag / Conjugate: This Periostin protein is labelled with His tag.

Application: Western Blotting (WB), ELISA

Specificity: The amino acid sequence of the recombinant human OSF-2 is 100 % homologous to the part of sequence 22-669 AA of the human OSF-2 without signal sequence.

Characteristics: Recombinant Human Periostin

Purification: Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

Purity: > 90 % (SDS-PAGE analyzed).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

Storage: -20 °C

Target Details

Target: Periostin (POSTN)

Alternative Name: Periostin (POSTN Products)

Synonyms: fa99h07 Protein, pn Protein, postn Protein, wu:fa99h07 Protein, wu:fc70f09 Protein, zgc:153873 Protein, LOC100008945 Protein, osf-2 Protein, pdlpostn Protein, periostin Protein, OSF-2 Protein, OSF2 Protein, PDLPOSTN Protein, PN Protein, RP11-412K4.1 Protein, A630052E07Rik Protein, AI747096 Protein, Osf2 Protein, PLF Protein, peri Protein, Plf Protein, periostin Protein, periostin, osteoblast specific factor b Protein, periostin, osteoblast specific factor Protein, POSTN Protein, postnb Protein, postn Protein, Postn Protein

Background: The Human OSF-2 His-Tagged Fusion Protein is produced in E. coli, molecular weight is 75 kDa protein containing 648 AA residues of the human OSF-2 and 23 additional AA residues - HisTag, Xa - cleavage site. Introduction: Periostin is a disulfide linked 90 kDa, 811 AA protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.