LMTK3 Protein (AA 133-411) (His tag)
LMTK3
Origin: Human
Host: Escherichia coli (E. coli)
> 95 %
AcA, CC
Active
2 images
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- Target See all LMTK3 Proteins
- LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))
- Biological Activity
- Active
- Protein Characteristics
- AA 133-411
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LMTK3 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.
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- Application Notes
- Isoelectric Point: 6.3
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))
- Abstract
- LMTK3 Products
- Synonyms
- LMTK3 Protein, LMR3 Protein, TYKLM3 Protein, BC059845 Protein, aatyk3 Protein, lemur tyrosine kinase 3 Protein, lmtk3 Protein, LMTK3 Protein, Lmtk3 Protein
- Background
- Alternative Names: LMR3, TYKLM3, Serine/threonine-protein kinase LMTK3
- Molecular Weight
- 46kDa
- UniProt
- Q96Q04
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