MMP13 Protein (AA 14-466) (His tag)

Catalog No. ABIN5665876

Product Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Biological Activity: Active

Protein Characteristics: AA 14-466

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This MMP13 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 97 %

Biological Activity Comment: Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant rat MMP13 was shown in figure 1 Gelatin hydrolysis by recombinant rat MMP13.

Application Details

Application Notes: Isoelectric Point: 5.1

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: Matrix Metalloproteinase 13 (MMP13) (MMP13 Products)

Synonyms: CLG3 Protein, MANDP1 Protein, Clg Protein, MMP-13 Protein, Mmp1 Protein, cb1034 Protein, mmp13 Protein, gene A Protein, xCol Protein, xcl3 Protein, MMP13 Protein, MGC108008 Protein, matrix metallopeptidase 13 Protein, matrix metallopeptidase 13a Protein, matrix metallopeptidase 13 (collagenase 3) like S homeolog Protein, matrix metallopeptidase 13 (collagenase 3) Protein, matrix metalloproteinase 13 Protein, matrix metallopeptidase 13 (collagenase 3) like L homeolog Protein, MMP13 Protein, Mmp13 Protein, mmp13a Protein, mmp13l.S Protein, mmp13 Protein, LOC100136348 Protein, mmp13l.L Protein

Background: Alternative Names: CLG3, Collagenase 3

Molecular Weight: 60kDa

UniProt: P23097