GDF9 Protein (AA 320-454) (His tag)

Catalog No. ABIN6239856

Product Details

Target: GDF9 (Growth Differentiation Factor 9 (GDF9))

Biological Activity: Active

Protein Characteristics: AA 320-454

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GDF9 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GDF9 with S100A8.

Application Details

Application Notes: Isoelectric Point: 7.1

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

References

Li, Meng, Liu, Li, Zhang, Zhou, Yao, Dong, Liu, Zhou, Li, Tao, Wu, Shen, Liu: "Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells." in: Development (Cambridge, England), Vol. 147, Issue 13, (2020) (PubMed).

Target Details

Target: GDF9 (Growth Differentiation Factor 9 (GDF9))

Abstract: GDF9 Products

Synonyms: GDF-9 Protein, zgc:123030 Protein, Gdf-9 Protein, growth differentiation factor 9 Protein, GDF9 Protein, gdf9 Protein, Gdf9 Protein

Molecular Weight: 18kDa

UniProt: O60383