PLG Protein (AA 191-433) (His tag)

Catalog No. ABIN5665893

Product Details

Target: PLG (Plasminogen (PLG))

Biological Activity: Active

Protein Characteristics: AA 191-433

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PLG protein is labelled with His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Plg with ACTb.

Application Details

Application Notes: Isoelectric Point: 7.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: PLG (Plasminogen (PLG))

Abstract: PLG Products

Synonyms: wu:fb70e09 Protein, PLG Protein, LPA Protein, plg Protein, Ab1-346 Protein, AI649309 Protein, Pg Protein, plasminogen Protein, plg Protein, PLG Protein, Plg Protein

Background: Alternative Names: PL, Plasmin, Activation peptide, Angiostatin

Molecular Weight: 38kDa

UniProt: Q01177

Pathways: Complement System, Lipid Metabolism