MSTN Protein (AA 267-375) (His tag)
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- Target See all MSTN Proteins
- MSTN (Myostatin (MSTN))
- Biological Activity
- Active
- Protein Characteristics
- AA 267-375
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MSTN protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of MSTN with BMP1.
- Top Product
- Discover our top product MSTN Protein
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Myostatin (MSTN) (Active) protein
MSTN Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Myostatin (MSTN) (AA 267-375) protein (His-IF2DI Tag)MSTN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Myostatin (MSTN) (AA 1-375) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myostatin (MSTN) (AA 121-202) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Myostatin (MSTN) (AA 241-310) protein (GST tag)MSTN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Isoelectric Point: 6.8
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4.
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- Target
- MSTN (Myostatin (MSTN))
- Abstract
- MSTN Products
- Synonyms
- GDF-8 Protein, GDF8 Protein, gdf8 Protein, mstn Protein, zgc:109749 Protein, Gdf8 Protein, NV13143 Protein, MSLHP Protein, Cmpt Protein, myostatin Protein, myostatin b Protein, growth/differentiation factor 8 Protein, MSTN Protein, mstnb Protein, LOC5577632 Protein, BRAFLDRAFT_290840 Protein, Mstn Protein, mstn Protein
- Background
- Alternative Names: GDF8, Growth Differentiation Factor 8
- Molecular Weight
- 14kDa
- UniProt
- O14793
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