EPO Protein (AA 30-193) (Fc Tag)

Catalog No. ABIN6253353

This Recombinant EPO protein is produced in CHO Cells.

Quick Overview for EPO Protein (AA 30-193) (Fc Tag) (ABIN6253353)

Target: EPO (Erythropoietin (EPO))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 30-193

Purification tag / Conjugate: This EPO protein is labelled with Fc Tag.

Purpose: EPO (human):Fc (human) (rec.) (non-lytic)

Specificity: The extracellular domain of human EPO (aa 30-193) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Characteristics: Protein. The extracellular domain of human EPO (aa 30-193) is fused to the N-terminus of the Fc region of a mutant human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Erythropoietin is the principal hormone involved in the regulation of erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells and the maintenance of a physiological level of circulating erythrocyte mass. It is a glycoprotein produced primarily by the kidney. The biological effects of EPO are mediated by the erythropoietin receptor (Epo R). Genetic variations in EPO is associated with susceptibility to microvascular complications of diabetes type 2 (MVCD2) (including diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease and diabetic neuropathy). It is used for the treatment of anemia and misused as a performance-enhancing drug in endurance athletes.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by the dose-dependant stimulation of human megakaryoblastic leukemia cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO

Target Type: Hormone

Background:

Alternate Names/Synonyms: Erythropoietin, Epoetin

Product Description: Erythropoietin is the principal hormone involved in the regulation of erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells and the maintenance of a physiological level of circulating erythrocyte mass. It is a glycoprotein produced primarily by the kidney. The biological effects of EPO are mediated by the erythropoietin receptor (Epo R). Genetic variations in EPO is associated with susceptibility to microvascular complications of diabetes type 2 (MVCD2) (including diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease and diabetic neuropathy). It is used for the treatment of anemia and misused as a performance-enhancing drug in endurance athletes.

NCBI Accession: NP_000790

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity