CXCL12 Protein (AA 22-89) (Fc Tag)

Catalog No. ABIN6253399

Product Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Protein Characteristics: AA 22-89

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CXCL12 protein is labelled with Fc Tag.

Purpose: SDF-1alpha [CXCL12] (human):Fc (human) (rec.)

Specificity: The extracellular domain of human SDF-1alpha (aa 22-89) is fused to the N-terminus of the Fc region of human IgG1.

Characteristics: Protein. The extracellular domain of human SDF-1alpha (aa 22-89) is fused to the N-terminus of the Fc region of human IgG1. Source: HEK 293 cells. Endotoxin content: <5EU/mg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine that belongs to the CXC chemokine subfamily. SDF1 is widely expressed in various organs including heart, liver, brain, kidney, skeletal muscle, and lymphoid organs, and is highly conserved between species. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1alpha and SDF1beta, which are identical except for the four residues present in the C-terminus of SDF1beta but absent from SDF1alpha. As a highly efficacious lymphocyte chemoattractant, SDF1 activates and directs the migration of leukocytes in response to proinflammatory stimuli such as LPS, TNF, or interleukins. It has been demonstrated that SDF1 promotes tumor growth and malignancy, enhances tumor angiogenesis, participates in tumor metastasis, and thus plays an important role in carcinoma pathogenesis. In addition, SDF1 is identified as the biological ligand for CXC-chemokine receptor 4 (CXCR4), which is widely expressed in leukocytes and acts as the cofactor for HIV-1 entry, and inhibits the HIV-1 infection. Furthermore, SDF1 is rapidly inactivated via N-terminal processing by cathepsin G associated with the membrane of B-cells, NK cells and to a lesser extent, T-cells.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <5EU/mg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: SDF-1alpha [CXCL12] (CXCL12 Products)

Synonyms: IRH Protein, PBSF Protein, SCYB12 Protein, SDF1 Protein, TLSF Protein, TPAR1 Protein, CINC-1 Protein, Gro1 Protein, Pbsf Protein, Scyb12 Protein, Sdf1 Protein, Tlsf Protein, Tpar1 Protein, Fsp Protein, KC Protein, Mgsa Protein, N51 Protein, Scyb1 Protein, gro Protein, sdf-1 Protein, sdf1 Protein, xSDF-1 Protein, SDF-1 Protein, cxcl12 Protein, sdf-1a Protein, sdf1a Protein, wu:fa55e10 Protein, wu:fc16h12 Protein, wu:fj84c02 Protein, C-X-C motif chemokine ligand 12 Protein, C-X-C motif chemokine ligand 1 Protein, chemokine (C-X-C motif) ligand 12 Protein, chemokine (C-X-C motif) ligand 1 Protein, C-X-C motif chemokine ligand 12 L homeolog Protein, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) Protein, CXCL12 Protein, cxcl12 Protein, Cxcl1 Protein, Cxcl12 Protein, cxcl12.L Protein, cxcl12a Protein

Background:

Alternate Names/Synonyms: CXCL12, C-X-C Motif Chemokine 12, PBSF, SCYB12, TLSF, TPAR1

Product Description: The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine that belongs to the CXC chemokine subfamily. SDF1 is widely expressed in various organs including heart, liver, brain, kidney, skeletal muscle, and lymphoid organs, and is highly conserved between species. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1alpha and SDF1beta, which are identical except for the four residues present in the C-terminus of SDF1beta but absent from SDF1alpha. As a highly efficacious lymphocyte chemoattractant, SDF1 activates and directs the migration of leukocytes in response to proinflammatory stimuli such as LPS, TNF, or interleukins. It has been demonstrated that SDF1 promotes tumor growth and malignancy, enhances tumor angiogenesis, participates in tumor metastasis, and thus plays an important role in carcinoma pathogenesis. In addition, SDF1 is identified as the biological ligand for CXC-chemokine receptor 4 (CXCR4), which is widely expressed in leukocytes and acts as the cofactor for HIV-1 entry, and inhibits the HIV-1 infection. Furthermore, SDF1 is rapidly inactivated via N-terminal processing by cathepsin G associated with the membrane of B-cells, NK cells and to a lesser extent, T-cells.

Molecular Weight: ~35kDa (SDS-PAGE)

NCBI Accession: NP_954637

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling