HMGB1 Protein (AA 24-215) (His tag)

Catalog No. ABIN6253402

This Recombinant HMGB1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for HMGB1 Protein (AA 24-215) (His tag) (ABIN6253402)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 24-215

Purification tag / Conjugate: This HMGB1 protein is labelled with His tag.

Purpose: HMGB1 (human) (rec.) (His)

Specificity: Human HMGB1 (aa 24-215) is fused at the N-terminus to a His-tag.

Characteristics: Protein. Human HMGB1 (aa 24-215) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg protein (LAL test, Lonza). Lyophilized from a concentrated sterile solution containing 50  mM Tris-Hcl buffer (  pH 8.0) and 100  mM NaCl. Purity: >90 % (SDS-PAGE). HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.

Endotoxin Level: <0.1EU/μg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from a concentrated sterile solution containing 50  mM Tris-Hcl buffer (  pH 8.0) and 100  mM NaCl.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background:

Alternate Names/Synonyms: High Mobility Group Protein B1

Product Description: HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.

NCBI Accession: NP_002119

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome