NAMPT Protein (AA 1-491) (DYKDDDDK Tag)

Catalog No. ABIN6253488

Product Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-491

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NAMPT protein is labelled with DYKDDDDK Tag.

Purpose: Nampt (mouse) (rec.) (enzymatically active)

Specificity: Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

Characteristics: Protein. Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg purified protein (LAL test, Lonza). Liquid. Contains PBS. Purity: >90 % (SDS-PAGE). Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.

Purity: >90 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment: 0.5μg to 5μg of Nampt (mouse) (rec.) (enzymatically active) show activity in a colorimetric Nampt assay.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Contains PBS.

Handling Advice: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt (NAMPT Products)

Synonyms: 1110035O14Rik Protein, PBEF Protein, PBEF1 Protein, VF Protein, VISFATIN Protein, AI314458 Protein, AI480535 Protein, NAmPRTase Protein, Pbef Protein, Pbef1 Protein, Visfatin Protein, visfatin Protein, pbef Protein, pbef1 Protein, nicotinamide phosphoribosyltransferase Protein, Nicotinamide phosphoribosyltransferase Protein, nicotinamide phosphoribosyltransferase S homeolog Protein, NAMPT Protein, Nampt Protein, nampt Protein, Smon_0680 Protein, Mrub_2845 Protein, Cseg_3704 Protein, BC1002_3997 Protein, Ftrac_2686 Protein, Varpa_5681 Protein, Celal_3900 Protein, Deima_0880 Protein, BC1001_3724 Protein, Celly_1696 Protein, Clole_1542 Protein, Tsp_05934 Protein, nampt.S Protein

Background:

Alternate Names/Synonyms: Visfatin, PBEF, Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase, NAmPRTase

Product Description: Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is the rate-limiting enzyme in the nicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. In addition it has been shown to be an adipokine that is localized in the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. The recombinant Nampt (mouse) (rec.) (enzymatically active) (Prod. No. AG-40B-0179) shows activity to convert nicotinamide + phosphoribosyltransferase (PRPP) to nicotinamide mononucleotide (NMN). It migrates as a homodimer on a size exclusion chromatography (SEC) column.

UniProt: Q99KQ4