PRKAB1 Protein (His tag)
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- Target See all PRKAB1 Proteins
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PRKAB1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product PRKAB1 Protein
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Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 62-269) protein (His-IF2DI Tag)
PRKAB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 1-270) protein (GST tag)PRKAB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 2-270) protein (His tag)Crystallography grade PRKAB1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (AA 2-270) protein (His tag)Crystallography grade PRKAB1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) protein (Myc-DYKDDDDK Tag)PRKAB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: 5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))
- Alternative Name
- PRKAB1 (PRKAB1 Products)
- Synonyms
- AMPK Protein, HAMPKb Protein, 1300015D22Rik Protein, AU021155 Protein, E430008F22 Protein, MGC82489 Protein, prkab1 Protein, wu:fk93d05 Protein, wu:fw87e09 Protein, zgc:56652 Protein, zgc:76975 Protein, zgc:92228 Protein, protein kinase AMP-activated non-catalytic subunit beta 1 Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b Protein, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a Protein, PRKAB1 Protein, Prkab1 Protein, prkab1.S Protein, prkab1 Protein, prkab1b Protein, prkab1a Protein
- Background
- 5'-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 32.8 kDa (293aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_006244
- Pathways
- AMPK Signaling, Warburg Effect
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