RTCD1 Protein (AA 1-366) (His tag)

Catalog No. ABIN5778793

Product Details

Target: RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-366

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RTCD1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))

Alternative Name: RTCA (RTCD1 Products)

Synonyms: Dmel\\CG4061 Protein, EG:22E5.3 Protein, RTC1_DROME Protein, DKFZp469J2025 Protein, cb158 Protein, rtcd1 Protein, sb:cb158 Protein, wu:fc62c03 Protein, zgc:56339 Protein, RTCD1 Protein, RPC Protein, RTC1 Protein, 2310009A18Rik Protein, AI450277 Protein, Rtcd1 Protein, RNA 3'-terminal phosphate cyclase Protein, Rtca Protein, RTCA Protein, rtca Protein, rtca.L Protein

Background: RTCA catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP, (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A, (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 41.7 kDa (389aa) confirmed by MALDI-TOF

NCBI Accession: NP_003720

UniProt: O00442